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A set of five SBSTDG generators is considered and the aim is to assist a hypothetical test engineer in elucidating good generators to fulfil the branch testing of a given programme.
The program WeightLESS [ 6], originally written to allow for interior branch testing using the WLS likelihood ratio test, has been modified to calculate also the P-values corresponding to each topology in the tree input file.
Although the phylogenetic distances are not independent because taxa share common evolutionary history, our previous results suggest that this does not affect the performance of the WLS method for branch testing [ 6].
Although the phylogenetic distances are a priori not independent because taxa share evolutionary history, we have shown in a previous work that ignoring the covariances does not have drastic consequences for the reliability or the accuracy of the LS approach to interior branch testing in phylogenetic trees derived from sequences [ 6].
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Internal branch topology was validated via the interior branch test.
Internal Branch Tests were conducted using 1000 replicates.
The branch tests applied above use an ω value averaged across the entire gene.
Note that interior branch test is more reliable for closely related sequences [33].
However, a branch test did show that it is significantly larger than ω along the background branches (p = 0.011, model 2 free ω vs. model 0).
The branch test did not indicate that ω along the foreground branch is significantly larger than 1 (model 2 free ω vs. model 2 ω = 1).
Inclusion of env V1-V2 domains coupled with internal branch tests for positive selection in our analysis was critical to uncover episodic selection within HIV-1 quasispecies.
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