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The radius of branch segment "5" is twice that of branch segment "6".
Branch segment "1" has the largest radius, equal to 160 μm and eight times that of branch segment "6".
The radius of branch segment "2" is six times that of "6".
In addition, branch segment length (the distance from one branch point to the next) for normal PC was constant at 5.5 ± 0.3 μm for all branches except the first branch segment (Fig. 4b).
The radius of each branch segment takes one of the five values as the two-dimensional case.
We measured the branch angles and branch segment lengths of PC in 3-D space (Fig. 4a, b).
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Firstly, partially visible branch segments within the tree canopies were connected using morphological features of the segments to form whole branches.
The length and orientation of branch segments were then analysed to link individual sections of the same branch together and to represent the branches with an equation.
Linear and logarithmic model equations were fitted to the branch segments and the equation with minimum residual was selected as the best-fit model representing the corresponding branch.
Quantitative analysis of the dendritic branch segments demonstrated that the average number, diameter and volume gradually diminished from proximal to distal segments.
We found that over-expression of the NR3B receptor subunit increased the length and complexity of dendritic arbor, and increased numbers of dendritic filopodia, suggesting that NR3B promotes the addition of branch segments in developing motor neurons.
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