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(E) The number of segments in total and by branch order.
Golgi-Cox analysis of dendritic branch order, dendritic length, and spine density demonstrate that an early mTBI increases complexity of pyramidal neurons in the mPFC.
Dendritic architecture (dendritic length, branch order, and spine density) along with cell quantification (neuron and glia) was generated for layer 3 pyramidal cells of the medial prefrontal cortex (mPFC/Cg3), orbital prefrontal cortex (OFC/AID), and CA1 of the hippocampus.
MSNs and type II branching probabilities could be accurately modelled with decreasing functions of both the metrical (exponential functions) and topological (power functions of the centrifugal branch order) distances to soma.
The cul3 neurons also exhibited additional, new branch order.
NeuronStudio [62] was used to measure total dendritic length and analyze neuron branch order.
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(D) The Sholl analysis of dendritic complexity by branch order-primary, secondary and tertiary branches.
In particular, we detected additional branch orders as well as additional branching in each order (Table 2).
We calculated branch orders for the ddaA, ddaB and ddaF neurons (Table 2).
Specifically, all observed cul1-mutant ddaC neurons had no more than 5 branch orders.
We detected that a typical wild type ddaC neuron contains 6 branch orders (Table 1).
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