However the coordination can be extremely complex and circuitous, i.e. genes may be involved in a multi-branch feedback loop with several TFs made or activated/deactivated by the proteins they encode.
Therefore 112 students would be required to detect the main effects and large interaction effect of branching and feedback on self-reported scores.
The pathway involves branching and feedback connections that act to ensure process such as H2BK123Ub and histone acetylation not only occur during transcription, but are also transient.
Using tools from robust control theory, we show that the limitations of renewal control can be circumvented through the introduction of lineage branching, with feedback promoting alternate fate choice at the expense of both primary fate choice and self-renewal.
If the interaction between branching and feedback interventions is of same order of magnitude as the expected main effects then we would require a fourfold increase in the sample size to give a total of 352 students.
The primary analysis will investigate the fixed effects of the factorial combinations of branching and feedback on the primary outcome measures, performance in a standardised composite clinical reasoning assessment and a 15-item self reported evaluation.
Assuming the effect size to be the same for both branching and feedback interventions, a sample size of 88 students would provide sufficient power to detect the main effects and an interaction effect that was twice as large as the assumed main intervention effect in the setting shown in Table 2.
Given unforeseen recruitment problems and some loss to follow-up, a target of 112 students should be easily achievable to quantify the main intervention effects (branching and feedback) which are the primary focus of the study, with increasing recruitment above this target providing increasing power to detect potential interactions between the main effects.
For each of the stages, input branches, loop filters, and feedback branches were modeled as potential mismatch sources.
The four case designs are: A) not branched+ no-feedback; B) branched+ no feedback; C) not branched+ feedback; D) branched+ feedback.
The conventional SIC detector is the standard SIC with RAKE receivers employed at each stage and the multi-branch multi-feedback SIC (MB MF-SIC) detection algorithm mentioned in [25] is presented here for comparison purposes.
