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The second fully expanded leaves were harvested at the secondary branch differentiation stage, immediately frozen in liquid nitrogen and stored at -80°C.
The second fully expanded leaves were harvested at the secondary branch differentiation stage, immediately frozen in liquid nitrogen and stored at −80°C until use.
Analysis disclosed that 29,064–29,928 genes at the secondary branch differentiation stage were expressed in the three F1 hybrids and their parents (Additional file 1: Table S1).
A strict statistical cut-off for SNP calling enabled fully quantitative analyses of both overall and allele-specific gene expression profiles to be obtained for rice leaves at the stage of secondary branch differentiation.
Heterosis levels were evaluated by measuring the fresh and dry biomass of the above-ground parts of the plants at the secondary branch differentiation stage, which is critical for determining the highest biomass and maximum grain yield.
When the inflorescences had developed to the secondary branch differentiation stage, the rice plants were divided into two groups for LD (15-h light/9-h dark) and SD treatment, respectively.
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Most of the development occurs postnatally, when the gland undergoes a highly regulated cascade of invasive growth, branching, differentiation, secretion, apoptosis and remodelling during each pregnancy cycle [ 1, 2].
The unique signature of the [Ca2+]c change determines the specificity in the signaling response that regulates a wide range of processes like hyphal tip growth, branching, differentiation, cell cycle, stress response and virulence [14 16].
Recent findings suggest notch signaling activation can contribute to generation and activation of microglial cells and astrocytes, inhibition of neurite growth, more dendritic branching, differentiation of oligodendrocyte progenitors and demyelination in both peripheral nervous system and CNS [ 9, 18- 24].
In addition to the familiar linear branched differentiation diagrams, it may also be useful to describe four major subsets of CD4+ T cells Th1, Th2, Th17, and Treg cells each of which can take on characteristics of the other subsets, or further characteristics.
Understanding the establishment and maintenance of polarity, hyphal elongation, septation, branching and differentiation are at the core of current research.
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