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After staining, brains were washed three times with PT and mounted in 80% glycerol (in PT).
After autopsy, the brains were washed in ice-cold saline and dissected into different regions based on standard anatomical markings.
Brains were washed in 0.1 M phosphate buffer before cutting free-floating 70 µm thick coronal sections on a vibratome.
Animals were sacrificed, and the brains were washed with ice-cold 0.01 M sterilized phosphate buffered solution (PBS).
Before applying the secondary antibodies for one day at 4°C, brains were washed six times with PBT.
Stained brains were washed in PBS, dehydrated twice in methanol, and cleared in BABB (benzyl alcohol/benzyl benzoate 1∶2) for photography [30].
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But it does more than that: it gives a shape to a process that we then might easily take for granted: can brains be "washed"?
The sections from the entire brain were washed in PBS-0.2% Triton and treated for 30 min with H2O2 (0.3%).
For brain sections: sections from the entire brain were washed in PBS-0.2% Triton and blocked with goat serum (1 100 in PBS, Vector) for 60 min.
The excised brain was washed in ice-cold saline and subsequently, one hemisphere was homogenized in 12 mL ice-cold saline and centrifuged for 5 min at 4,000 rpm.
After incubation, the brain was washed three times with 5 min intervals in PBS.
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