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In brief, whole brains were treated for silver impregnation for 2 weeks.
To obtain pure protein lysate, mouse brains were treated as described above.
After permeabilization with PBS containing 2% Triton X-100, the brains were treated with 70% formic acid (Sigma), and stained with a mouse monoclonal anti-Aβ antibody (Chemicon) followed by detection with biotin-XX goat anti-mouse IgG and streptavidin-Oregon Green 488 conjugate (Molecular Probes).
Next, brains were treated with Proteinase K (0.05 μg/μl, 50 mM Tris HCl, 50 mM EDTA) for 1 h.
Paraffin sections of AD brains were treated with 10 μg/mL Proteinase K (Pro-K) for 30 min after autoclaving (Ac) and formic acid (FA) treatment.
After fixation, mouse brains were treated for 1.5 hours in 98% formic acid to reduce the infective titer for safety reasons.
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Following fixation in 10% formal saline for 48 h, the mouse half-brains were treated for 1.5 h in 98% formic acid, cut transversely into four sections, and embedded in paraffin.
Tissue sections from human AD brain were treated with cold (+4°C) 99% formic acid for 1 minute at RT (25°C).
In our series, 5 out of 13 patients with an isolated relapse in the brain were treated with ICE chemotherapy, of which three patients achieved a long-term complete response.
Finally, to assess how the data suggesting that inhibition of LRRK2 induces autophagy in model cell lines relates to primary cells, primary astrocytes isolated from rat brain were treated with LRRK2-in1.
Stroke caused by intracranial aneurysms or AVMs bleeding into the brain are treated in various ways.
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CEO of Professional Science Editing for Scientists @ prosciediting.com