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Whole brains were processed in all murine cases.
Age-matched naïve and sham-operated tgAPP21 rats served as controls (n = 19) and their brains were processed in exactly the same way.
This was not due to variation in the immunohistochemical procedure as the brains were processed in groups of four, each of which contained one female and one male brain of each genotype.
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Further sections of the brain were processed in TUNEL procedure to detect evidence of apoptotic cells.
In a person who is conscious, he explained, information entering the brain is processed in a few areas and then distributed or broadcast to many others.
In a person who is conscious, he explained, information entering the brain is processed in a few areas and then distributed — or broadcast — to many others.
Each brain was processed in one paraffin block and three sections were cut and stained with hematoxylin and eosin (H/E), Gram, and napthol esterase to identify mast cells.
The mouse embryos were obtained from pregnant females, and their brains were processed either for in situ hybridization, immunohistochemistry or for preparing organotypic cultures for in vitro migration assays.
In addition, brains were processed using Golgi impregnation to assess the effects of sexual experience on dendritic spines and dendritic complexity in the hippocampus.
Brains were processed for paraffin embedding and cut in 8 μm coronal sections with every 10th section mounted on a SuperFrost (VWR, Lutterworth, UK) glass slide.
A total of 240 samples from 29 matched cortical and subcortical regions in two brains were processed using Illumina HiSeq RNA-Seq technology.
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