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PBS-perfused brains were postfixed overnight in 4% formalin in PBS at 4°C.
The brains were postfixed overnight in 4% PFA, cryoprotected in 30% sucrose solution (0.01 M PBS) and embedded in OCT.
Brains were postfixed overnight and equilibrated in 30% sucrose.
Dissected brains were postfixed overnight and further cryoprotected overnight with 30%% sucrose.
The excised brains were postfixed overnight and then equilibrated in 30% sucrose solution for 1 day.
Brains were postfixed overnight and cryoprotected in increasing gradients of sucrose (10, 20 and 30%).
Similar(48)
The brain was postfixed overnight in 4% paraformaldehyde in PBS, pH 7.2, cryoprotected with 15%and25%5% sucrose solutions (subsequently overnight), frozen in 2-methylbutane (30 seconds at −42°C) and transferred to dry ice.
The collected brain tissues were postfixed overnight in the same fixative solution.
The brains were dissected from the skulls and were postfixed overnight.
Embryos were postfixed overnight in 4%PFA and stored in 50% glycerol.
The brains were postfixed in paraformaldehyde overnight and transferred to 30% sucrose in PBS/0.1% azide solution at 4°C for 2 3 days.
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