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Brains were mounted on sectioning stages with cyanocacrylic glue.
Brain sections obtained from perfused brains were mounted on slides and were stained with Cresyl Violet.
Frozen brains were mounted and placed in a cryostat and 7 µm sections were cut in the coronal plane.
Stained larvae brains were mounted in 70% glycerol, 30% Tris pH 7.6 and viewed using bright-field microscopy (Nikon, Eclipse E600).
Frozen brains were mounted in a cryostat microtome and 18 µm sections were cut with a knife temperature of -14°C and a sample temperature of -16°C.
Brains were mounted on slides using 50% glycerol or FluoroGaurd™ anti-fade reagent Bio-Rad Laboratoriess, Hercules, CA) with the ventral side facing upward.
Similar(29)
Thereafter, the rats were sacrificed, and the lumbar spinal cord and the brain were mounted in Tissue-Tec OTC embedding compound, and deep frozen.
The brain was mounted and coronal slices (500 µm for rat, 250 µm for mice) were cut using a vibratome.
The cerebellum and frontal cortex were removed and the brain was mounted for coronal slicing.
The brain was mounted with the ventral surface uppermost and placed in a vibrating microtome (Microfield Scientific, Dartmouth, UK).
A series of every sixth section through each brain was mounted onto gelatin-chrome-coated Superfrost microscope slides (VWR, Radnor, PA).
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