Sentence examples for brains were lysed from inspiring English sources

Dissected mouse brains were lysed in Triton lysis buffer (10 mM Tris pH 7.5, 50 mM NaCl, 1% Triton Tx-100, 1% aprotinin, 1 mM AEBSF, 1 mM sodium orthovanadate, 50 mM NaF, 25 mM β-glycerophosphate) and submitted to serial passages through 18G, 20G, and 25G needles.

Brains were lysed and P2 mitochondria-enriched fractions prepared.

BXD25 and BXD40 mouse brains were lysed directly in radioimmunoprecipitation (RIPA) buffer for analysis of whole cell lysates.

Mouse brains were lysed in 2% SDS/50 mM Tris pH 7.5 by sonication and heated to 100°C for 10 minutes.

Mouse brains were lysed in RIPA buffer (50 mM Tris-Cl, pH8.0, 150 mM NaCl, 1% NP-40, 0.5% Na-Doc, 0.1% SDS) containing protease inhibitors (Sigma-Aldrich).

EsiRNAi-transfected HeLa and mouse brains were lysed in a lysis buffer (50 mM HEPES pH = 7.5, 150 mM KCl, 1 mM MgCl2, 10% glycerol, 0.1% NP-40) with a protease inhibitor cocktail (Complete, Roche), resolved in SDS-PAGE, and transferred onto a nitrocellulose membrane.

Adult C57Bl6 mice were decapitated and whole brain was lysed in buffer A (50 mM Hepes pH 7.6, 150 mM NaCl, 10% glycerol, 1% triton x-100, 1 mM PMSF, 5 μg/ml leupeptin and 10 μg/ml aprotinin) and cleared by centrifugation.

Frozen tumor and normal brain tissues were lysed in RIPA lysis buffer with protease inhibitors as described [34].

Before analysis, brain homogenates were lysed in 1x Luciferase cell culture lysis reagent (Promega, Madison, WI, USA) for 15 min on ice and centrifuged at 14000 g for 5 min to collect the supernatants to be used in the assay.

N2a cells and brain tissue were lysed in RIPA buffer and denatured in Laemmli.

The brain slices were lysed, and total RNA was extracted using the RNeasy Lipid Tissue kit (Qiagen, France) according to the manufacturer's instructions.