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In control experiments, rat brains were injected with PKH 26-labeled UCM cells that had been lysed by repeated sonic disruption.
Oxytocin's power was first recognised in 1979 when virgin female rats whose brains were injected with the hormone began to display maternal behaviour.
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The lateral ventricles of an embryonic brain were injected with plasmid DNA (2 μg/μl).
Using an intracranial glioma-bearing mouse model, we have demonstrated that luciferase activities were higher in actively proliferating glioma tumours than the corresponding post-mitotic normal brain parenchyma when both tumour and normal parts of the brain were injected with an equal amount of viruses.
A prominent reduction of iNOS expression was observed in the tumor regions of nude mice brains, which were injected with 5310 glioma cells, after MMP-9 and/or uPAR knockdown.
We treated each injection data set independently throughout the experiments though some of the brain regions were injected multiple times, since the specific injection voxels are unique.
Brain homogenates were injected with Nanofil syringes (World Precision Instruments, Sarasota, FL, USA) and steel bevel needles (32-gauge diameter) (World Precision Instruments) into the striatum at a rate of 0.25 μl/min with a total of 0.5 μl per mouse controlled with a pump (UltraMicroPump III with Micro4 pump controller, World Precision Instruments).
As an additional control to monitor the passage of FITC-labeled antibodies across the blood-brain barrier, mice were injected with FITC tagged β-syn.
Samples (brain homogenates and PBS) were injected into the right hippocampus using the following coordinates as measured from bregma: antero-posterior (AP), -1.8 mm; medio-lateral (ML), -1.8 mm; dorso-ventral (DV), -1.8 mm.
In other experiments brain tumour-bearing mice were injected with anti-IGFBP7-sdAb NP-Cy5.5 or non-targeted NP-Cy5.5 (10 mg Fe per kg body weight) via the tail vein.
To further analyze the state of iron deficiency in PrPKO brains, equal counts of 59FeCl3 were injected in the tail vein of Wt and PrPKO mice, and after a chase of 3 hours, mice were euthanized and cold PBS was infused from the heart to drain capillary blood.
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