Sentence examples for brains were frozen from inspiring English sources

The brains were frozen and 25 μm thick coronal frozen sections were cut with a sliding microtome (SM200R, Leica Biosystems, Wetzlar, Germany).

Brains were frozen whole (in situ) or tissue blocks immediately dissected and frozen (qRT-PCR).

For mice brain sections (20 µm), brains were frozen and cut with CryoStar NX70 cryostat (ThermoFisher Scientific).

After cryoprotection in 30% sucrose/PBS (pH 7.4), brains were frozen and serial coronal sections (60 μm sections) were cut with a microtome.

For in situ hybridisation, brains were frozen over dry ice, cryo-sectioned (12 µm), mounted onto poly-L slides, and stored at −80 °C.

After saturation with 30% sucrose solution, brains were frozen.

Brains were frozen in liquid isopentane at −70°C and stored at −80°C until sectioning.

Brains were frozen onto chucks with embedding medium (Tissue-Tek OCT; Fisher Scientific, Tustin, CA) and surrounded with powdered ice.

Fixed, cryoprotected brains were frozen and sectioned in the horizontal plane using a Cryostat (Leica), with sections collected serially.

Brains were frozen in mounting media (Tissue-Tek, ProScitech, Thuringowa, QLD, Australia) and 35 µm coronal sections were cut on a cryostat at −10°C.

Both hemi-brains were frozen on dry ice and kept at -80 °C before using.