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Brains were fixed immediately in 4% formalin and remained in this solution for 5 days.
Isolated E16.5 embryonic brains were fixed immediately for 24 h in 4% PFA in PBS at 4°C.
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In 10 mouse brains that were fixed immediately after death, they still found bacteria in similar locations.
For immunohistochemistry, the brain tumour specimens were fixed immediately in 4% phosphate-buffered formaldehyde and processed into paraffin blocks.
Briefly, brains were dissected in ice-cold PBS and were fixed immediately after dissection for 15 min at room-temperature in 4% paraformaldehyde (vol/vol).
Embryos were fixed immediately after the drug treatment (see below).
Hearts, livers, and kidneys were fixed immediately in 10% formalin.
LEEP and laser conisations were fixed immediately as described above.
After the mice were killed, their brains were fixed in 3.7% formaldehyde and immediately frozen.
Brains were fixed, sectioned and labeled with WGA.
Brains were fixed for 4 6 weeks.
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