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Animals were killed in adulthood (60 to 90 days of age), and their brains were fixed as above.
Brains were fixed as described for immunohistochemistry. Parallel series of 40-µm-thick coronal sections were obtained in a freezing microtome.
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Fly brains were fixed and stained as described [81].
Mouse brains were fixed and dissected as described above.
24 hr later, E14.5 pups were dissected and brains were fixed and processed as described above.
Larval brains were fixed and immunostained as previously described (Bello et al., 2007).
Neonate (2 7 days old) F1 transgenic mouse brains were fixed and stained as previously described (41).
For Congo Red fluorescence imaging, the brains were fixed and washed as above then stained with 0.2 mg/ml Congo Red in PBS for 10 min.
Third instar larval brains were fixed and stained in aceto-orcein as previously described (Rahmani et al., 2009).
Brains of control and hUCBSC-treated mice brains were fixed in formaldehyde and embedded in paraffin as per standard protocols.
Upon photoactivation, the brains were fixed and immunostained for tdTomato and tyrosine hydroxylase or dopa-decarboxylase as described above.
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