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For GAD1 immunostaining, the brains were fixed in Bouin's fixative.
Brains were fixed in Carnoy's fixative for 24 h, transferred to butanol and embedded in paraffin.
Alternatively, for immunofluorescence, brains were fixed in fresh 4% PFA overnight at 4 °C.
Brains were fixed in 4% PFA in PBS for 2 h and 30 min at 4 °C.
A survey found that many of them believed their brains were fixed at birth.
Brains were fixed, sectioned and labeled with WGA.
Brains were fixed in the same solution overnight.
Fly brains were fixed and stained as described [81].
Fly brains were fixed in 4% paraformaldehyde (PFA) in sodium phosphate buffer (PB; pH 7.4).
Brains were fixed in buffered formol for 12 hours and paraffin-embedded.
The brains were fixed in PFA (2%) and then embedded in paraffin.
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