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Their brains were extracted, postfixed for 24 hrs and then transferred to phosphate-buffered (0.1M) 30.0% sucrose solution in which they remained for a further 24hrs.
Their brains were extracted, postfixed for 24 h, and then transferred to 25% distilled water sucrose solution in which they remained for a further 24 h.
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Brains were extracted and postfixed 1 h in 4%% paraformaldehyde and then transferred into 0.1 M PB for sectioning.
Brains were extracted as described before, postfixed with 4% PFA and cryoprotected with 30% sucrose.
The brains were extracted from the skull and postfixed in the fixation solution for 12h, cryoprotected overnight in 30% sucrose, and then sectioned in coronal plane at a 40 μm thickness using a cryostat (Leica Microsystems, Germany).
The brains were extracted from the skull and placed on a stirrer to postfix in PFA for 4 h, after which the brains were placed in 25% sucrose overnight.
The brains were extracted from the skull and placed on a stirrer to postfix in PFA for four hours, after which the brains were placed in 25% sucrose overnight.
The brains were extracted from the skull and placed on a stirrer to postfix in PFA for 4 hr, after which the brains were placed in 25% sucrose overnight.
The brains were harvested, postfixed, and sectioned at 40 μm.
Brains were removed, postfixed for ∼8 h in 4% paraformaldehyde, and kept in PBS overnight.
The brains were removed, postfixed in 4% paraformaldehyde and paraffin embedded.
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