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Because of the reason, alternatively, we here strictly controlled time for brain extraction; mouse brains were extracted within 1 minute (typically 40 seconds) after sacrifice.
Their brains were extracted and halved.
Thirty minutes later, the animals were killed with carbon dioxide; the brains were extracted and snap frozen.
Brains were extracted and prepared for molecular assays of 1) apoptosis and 2) pro and anti-apoptotic proteins on day 7 of postnatal life.
Brains were extracted and stored overnight in 30% sucrose in 4% paraformaldehyde.
Brains were extracted, post-fixed overnight, and cryoprotected in 30% sucrose in PB.
Similar(10)
At the PND 60 animals were taken from their home cages and sacrificed, their brains was extracted, immediately frozen in isoproponol and stored at −80°C.
For further analysis RNA from 3.5 4 month old mouse brains was extracted using Trizol (Invitrogen).
RNA from LV-injected brains was extracted using a RNeasy Lipid Tissue kit (Qiagen).
Total RNA from brains was extracted using TRIzol reagent (Gibco-BRL, Life Technologies).
Total RNA from all brains was extracted following the same protocol used for RNA-sequencing preparations (see above).
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