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Exact(55)
Thereafter, the resin was changed once and brains were embedded in a single block at 60 °C to allow for polymerization of the resin.
Rat brains were embedded in resin and sectioned serially to allow quantitative 3-D analyses of single neurons or groups of neurons.
For immunostaining of glial fibrillary acidic protein (GFAP), polyglutamine (1C2, MW2 and MW5), ubiquitin, TATA-binding protein (TBP) and hematoxylin and eosin staining, brains were embedded in paraffin and 5 μm sections were cut using a microtome.
The brains were embedded in paraffin according to standard protocols.
Cryoprotected embryonic brains were embedded in Tissue-Tek O.C.T. compound (Sakura) and frozen in 2-methylbutane chilled with dry ice.
Brains were embedded in 2% agar and cut coronally, by Vibratome (Lancer, St . Louis MO) into 500 µm-thick slices.
Similar(5)
a, For evaluation of ischaemic brain tissues, 1-mm-thick serial coronal slices from the brain were embedded in paraffin.
The spinal cord and brain were embedded in paraffin and cut into serial 6-µm thick coronal slides.
Coronal sections (4 μm) through the brain were embedded in paraffin and stained with hematoxylin and eosin (H&E) for microscopic evaluation of neuronal damage.
Rat brains were fixed by transcardial perfusion with saline, followed by perfusion and immersion in 4% paraformaldehyde, and the brain were embedded in paraffin and cut into 5 μm sections.
Briefly, the brain was embedded in 4% oxidized agarose and cross-linked with a 0.2% sodium borohydrate solution (in 0.05 M sodium borate buffer, pH 9.0 9.5).
More suggestions(15)
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