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The brains were dissected, postfixed in PFA for 16 hours, and transferred to 18% sucrose in 0.1 M PB for at least 16 hours.
Brains were dissected, postfixed for 2 h, and subsequently stored at 4°C in PBS.
Brains were dissected, postfixed overnight at 4°C and transferred into 30% sucrose/PBS solution.
Brains were dissected, postfixed for 30 min at room temperature and transferred into 10% sucrose for cryoprotection.
The brains were dissected, postfixed for at least 48 h in 4% formalin solution and washed in 70% ethanol.
46 Brains were dissected, postfixed in 4% paraformaldehyde (PFA), perfused with 20% sucrose at 4°C for 24 h, frozen, and cryosectioned (10 μm).
Similar(52)
Brains, spinal cords, and muscles were dissected, postfixed in 4% PFA and embedded.
The brains, hearts, livers, and kidneys were dissected, postfixed in the same solution, cryoprotected with 30% sucrose in PBS for 48 h, and then frozen.
The brain and cervical spinal cord were dissected, postfixed in the same fixatives, and immersed overnight in PBS containing 30% sucrose.
Retinas were dissected, postfixed in 1 % PFA for 2 h at room temperature, washed with PBS, and permeabilized with PBS containing 1 % Triton-X-100 for 1 h.
Tissue was dissected, postfixed for 2 hours and cryoprotected in 30% sucrose overnight.
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