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Where indicated brains were dissected into striatum, hippocampus and midbrain.
Brains were dissected into several compartments: Tumor, ipsilateral hemisphere, contralateral hemisphere and midbrain.
Control or heat-shocked rat brains were dissected into cerebral cortex, hippocampus, midbrain, and spinal cord.
Brains were dissected into 1 mm thick coronal slices and immersion fixed in 4% paraformaldehyde in 0.1 M PBS overnight at 4°C.
Briefly, brains were dissected into 2.5% paraformaldehyde, 1% glutaraldehyde, 1% sodium metabisulfite in 0.1 M cacodylate buffer, pH 7.2, microwaved with a Pella research-grade oven (# 3450, with variable power controller #3430 and cold spot) with chilling plate at 18°C and power setting #2 for 2 min on/2 min off/2 min on/2 min off, and then fixed overnight on a shaker at 4°C.
Flies were anesthetized on ice, and brains were dissected into cool AHL.
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Fly brains were dissected in phosphate-buffered saline and placed into Hank's balanced salt solution (HBSS).
E16 mouse brains were dissected in PBS containing 0.6% glucose and the hippocampus were dissected out.
Fresh brains were dissected in 0.5× RNAlater (Ambion).
Embryonic brains were dissected in cold artificial cerebrospinal fluid.
Each brain was dissected into 16 areas: spinal cord, medulla oblongata, pons, mesencephalon, thalamus, hippocampus, cerebellar vermis, cerebellar medulla, cerebellar hemispheres, occipital cortex, temporal cortex, parietal cortex, striatum, frontal cortex, piriform lobe and olfactory bulbs.
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