Sentence examples for brains were cut with from inspiring English sources

After 4% PFA post-fixation, brains were cut with a vibratome (Leica microsystem) in serial free-floating coronal sections of 35-µm thickness.

The brains were cut with a cryostat in the coronal plane from the level of the tractus septopallio-mesencephalicus to the caudal end of the tuberal hypothalamus.

Brains were cut with a vibratome in coronal corticostriatal slices (250 µm) in ice-cold solution, whose composition was (in mM): 110 choline, 2.5 KCl, 1.25 NaH2PO4, 7 MgCl2, 0.5 CaCl25 25 NaHCO3, 7 glucose, pH = 7.4 bubbled with O2/CO2 (95/5%).

The brains were cut with a coronal section at the injection site, and the anterior part was mechanically disrupted and used for establishing cell cultures whereas the posterior part was formalin-fixed and used for paraffin sectioning.

For comparing gene expression patterns of the PRMS and DRMS, freshly dissected brains were cut with a vibratome (Leica, Wetzlar, Germany) in 100  μm sections, and the corresponding brain regions were isolated using a binocular microscope and shock frozen immediately after isolation.

The left and right halves of each brain were cut with a freezing microtome alternating in five 15 and one 25 µm parasagittal sections per hemisphere under RNAse-free conditions.

Embedded brains were cut coronally (300 μm) with a VT1000S vibratome (Leica, Nussloch, Germany) at 4°C.

Brains were cut into small pieces, washed with PBS, homogenized with 3 ml of 10% sarcosine in TEND (10 mM Tris-HCl pH 8, 1 mM EDTA, 130 mM NaCl, and 1 mM dithiothreitol) containing a protease inhibitors cocktail (Cat. N. 1-836-153; Roche, INdianapolis, IN, USA), then incubated on ice for 1 h and centrifuged at 22,000× g for 30 min at 4°C.

After the rats were sacrificed by pentobarbital sodium overdose and perfused with formalin, their brains were cut coronally at 30 μm and stained with cresyl violet.

Brains were cut into 30 µm sections with a cryostat freezing microtome.

The brains were cut into 60-μm thick sections with a vibratome.