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The right hemisphere was mounted in optimal cutting temperature medium (OCT, Sakura Finetek, Thatcham, UK) for immunohistochemistry. Brains were cut into 10 micron sections using a cryostat.
The brains were cut into four blocks between the forebrain (olfactory bulb) and cerebellum, embedded in paraffin, and cut into consecutive 7-μm sections.
The brains were cut into 280 or 300 μm slices with a vibratome (Leica VT1200S, Germany); slices were then incubated with ACSF (in mmol/L: NaCl 126, KCl 2.5, NaH2PO4 1.25, NaHCO3 26, D-glucose 25, MgSO4 2, CaCl2 2, pH 7.4, saturated with 95% O2 and 5% CO2) at room temperature for 1 h.
Brains were cut into 30 µm sections with a cryostat freezing microtome.
Brains were cut into slices of 250 µm in a cryostat.
Whole brains were cut into 70 µm sections on a Vibratome and stored in phosphate buffered saline (PBS).
Similar(14)
Coronal sections of the entire brain were cut into 40-μm sections using a cryostat.
Coronal sections of the brain were cut into 40 50 μM thickness using a vibrating microtome (VT1000S, Leica, Milton Keynes, UK).
Samples of liver, heart and brain were cut into small pieces using a surgical blade, weighed and stored at −80°C until use.
Each rat brain was cut into six sections and mouse brain into five sections.
In keeping with Vogt's method, each brain is cut into four or five chunks, which are encased in paraffin wax.
More suggestions(14)
nerves were cut into
cells were cut into
muscles were cut into
brains were embedded into
brains were collected into
brains were split into
brains were combined into
brains were transferred into
brains were put into
brains were divided into
brains were sectioned into
brains were dissected into
brains were dissociated into
brains were placed into
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