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These results suggested that the human SNCA protein levels in these blood specimens did not parallel the expression levels found in the brains from the same mice.
Immunoreactivity against MTs was elevated in both the white and gray matter of postmortem brains from the same MLD patients, particularly in astrocytes (see Fig 2B).
A direct comparison between common garden and wild brains from the same populations revealed a habitat-dependent effect: pond (but not marine) fish had relatively larger brains in the wild than in the common garden.
Trunk blood was collected for determination of ACTH concentrations (10 animals/condition) and dissected brains from the same animals were frozen on dry ice and stored at -80°C (to be used for expression profiling).
Poly A+ RNA was isolated from the frontal cortex of frozen post mortem human brains from the same 4 AD and 5 age-matched control subjects as used in our previous study using the same the extraction procedure [ 34].
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The accuracy of the registration was established by measuring displacement of the 80 landmarks in each registered brain from the same landmarks in the reference brain.
The results presented show the precipitation in five TRα1 GFP animals normalized against the corresponding wt brain from the same experiment.
3) Critically, postulated changes produced by death would be expected to occur uniformly, in all parts of the brain from the same individual.
Marcus writes that the brain results from the same pathways that make lungs, noting that "from a gene's-eye view, brains are just one more elaborate configuration of proteins".
The mixed effect model accounted for potential correlation in signal intensities that may arise from having multiple brain regions from the same subject as well as having multiple technical replicates for the same brain regions.
(D) Ex vivo autoradiography of a corresponding brain section from the same mouse.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com