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Four fetal brains from each treatment group were analyzed using RNA Hi-Seq sequencing and the differential expression of genes was determined by the edgeR package using NetworkAnalyst.
Brains from each animal were homogenized (20% w/v) in cold PBS (DNase I 250 µg/ml) in a blender and then passed through different size needles.
Glial Fibrillary Acidic Protein (GFAP) immunohistochemical staining was conducted on every 12th section as described above with the following alterations: Staining was done in large trays with a 96 well insert, allowing for 2 brains from each group to be stained simultaneously.
Four rat brains from each group were removed six days after aluminum administration was completed.
After Morris water maze test, 4 rat brains from each group were harvested.
Brains from each genotype were fixed with 4% PFA in PBS overnight at 4°C.
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It's also hard to distinguish critical structures within the brain from each other.
The brain from each rat was sampled from −2.80 to −4.52 behind bregma.
The figures represent cryosections of a mouse brain from each group as a representative of six animals from each group.
The other hemi-brain from each mouse was snap-frozen for biochemical assays.
The other hemi-brain from each mouse was sagitally sectioned on a cryostat apparatus at 10 μm.
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