Sentence examples for brains embedded in from inspiring English sources

Mice were anaesthetised and perfused intracardially with PBS followed by 4% paraformaldehyde and the brains embedded in paraffin or, for cryosectioning, were equilibrated successively in 12%, 16% and 18% sucrose in PBS for cryoprotection and then frozen on dry ice.

Immunohistochemistry was performed on serial freefloating sections cut at 40-µm thickness for hippocampus as well as for the subventricular zone and olfactory bulb, at -25°C in a cryostat from brains embedded in Tissue-Tek OCT (Sakura, Torrence, CA).

For immunostaining of brain sections, mice were intracardially perfused with 4% paraformaldehyde and brains embedded in paraffin for sagittal sectioning.

Each of the MultiBrain® sections cut from the block was a composite holding individual sections from each of the brains embedded in the block.

Thereafter, the resin was changed once and brains were embedded in a single block at 60 °C to allow for polymerization of the resin.

Rat brains were embedded in resin and sectioned serially to allow quantitative 3-D analyses of single neurons or groups of neurons.

For immunostaining of glial fibrillary acidic protein (GFAP), polyglutamine (1C2, MW2 and MW5), ubiquitin, TATA-binding protein (TBP) and hematoxylin and eosin staining, brains were embedded in paraffin and 5 μm sections were cut using a microtome.

The brains were embedded in paraffin according to standard protocols.

Cryoprotected embryonic brains were embedded in Tissue-Tek O.C.T. compound (Sakura) and frozen in 2-methylbutane chilled with dry ice.

Brains were embedded in 2.5% low melting point agarose dissolved in HBSS and rapidly cooled on ice.

After washing (4×10 min) in Tris-SMB buffer, brains were embedded in agarose and cut into 60 µm sections.