Sentence examples for brain were washed in from inspiring English sources

The sections from the entire brain were washed in PBS-0.2% Triton and treated for 30 min with H2O2 (0.3%).

For brain sections: sections from the entire brain were washed in PBS-0.2% Triton and blocked with goat serum (1 100 in PBS, Vector) for 60 min.

The excised brain was washed in ice-cold saline and subsequently, one hemisphere was homogenized in 12 mL ice-cold saline and centrifuged for 5 min at 4,000 rpm.

Subsequently, the brain was washed in 0.9% normal saline to remove the blood from the surface, put into a 25 mL glass beaker filled with saline, and maintained at 37 degrees Celsius.

After autopsy, the brains were washed in ice-cold saline and dissected into different regions based on standard anatomical markings.

Brains were washed in 0.1 M phosphate buffer before cutting free-floating 70 µm thick coronal sections on a vibratome.

Stained brains were washed in PBS, dehydrated twice in methanol, and cleared in BABB (benzyl alcohol/benzyl benzoate 1∶2) for photography [30].

Brains were dissected in 1X PBS and fixed in 4% paraformaldehyde (PFA)/PBST (1X PBS +0.2% Triton-X 100), for 20 minutes at RT. Brains were washed in PBST, blocked in 5% Normal Goat Serum (NGS) for 1 hour at 4°C and incubated in primary antibody overnight at 4°C.

Brains were washed in PBS-TX at room temperature (about 22°C) and incubated with fluorophore-tagged secondary antibodies (Cy2- or Cy3-tagged IgGs, raised in goat; Jackson ImmunoResearch) diluted 1∶1000, either overnight at 4°C or 2 h at room temperature (around 22°C).

To prepare brain cells, mouse brains were washed in PBS and minced in dissociation buffer.

Brains were washed in phosphate-buffered saline (PBS) (with Ca++/Mg++) and meninges were thoroughly peeled off and discarded.