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The hippocampi from each brain were traced using a software program (Tracer) (Woods, 2003), available at http://www.loni.ucla.edu/Software/Software_Detail.jsp?software_id = 10.
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Thirty-six developing brains with well-labeled axons were completely analyzed microscopically and 24 representative brains were traced using a Neurolucida (25×) for more detailed morphological and statistical analysis, including ages (injected-recovered) P0 1 (n = 7), P2 3 (n = 4), P4 5 (n = 3), P6 7 (n = 4), P8 9 (n = 3).
All lesions were traced using the Analysis of Brain Lesions software package [7] and normalized in Talairach space to the Damasio's template [8].
MI borders were traced using these images.
Dendritic processes were traced using Optimas 6.0 image analysis software.
Lamellipodia were traced using the ImageJ software.
Backbone assignments were traced using SPARKY.
All axons within an image were traced using ImageJ software.
Neurons were traced using NeuronJ imageJ plugin (60).
The region of interest was traced using the × 1.25 objective.
Aortic contour was traced using a visually applied ellipse function.
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