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Primary cells from P2 mouse brain were stained with 25 μmol/L CFSE (107 cells/mL) for 20 min at 37°C.
Subsequently, samples were embedded in paraffin, and 6 µm frontal sections prepared from the entire brain were stained with hematoxylin-eosin.
To confirm that a significant reduction in myelin occurred in the corpus callosum of mice on the cuprizone diet at 4 weeks, and to examine the extent of remyelination at 14 days following cuprizone withdrawal and rhGas6 or PBS treatment, multiple non-serial coronal sections of mouse brain were stained with myelin basic protein (MBP) monoclonal antibody, SMI99.
Paraffin sections of brain were stained with H&E.
The tissues of heart, liver, spleen, lung, kidney and brain were stained with H&E.
Immunocytochemistry – Acetone fixed impression smears of pleura and brain were stained with a fluorescein conjugated commercial monoclonal antibody to Chlamydia group specific lipopolysaccharide (Imagen, Oxoid catalogue no K610111-2) and examined with a fluorescent microscope.
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Every 4th section of ON, SC, and brain was stained with LFB.
After TTC staining, the infarcted brain tissue appears white, whereas metabolically active areas of the brain are stained with a dark red precipitate.
Brains were stained with Masson's trichrome, hematoxylin and eosin, diaminobenzidine-enhanced Perls, and Gram stainings.
Brains were stained with either the anti-human p97 monoclonal antibody, L235 [55] or the rabbit anti-human Tf antibody (Research Diagnostics).
Tissue sections from the left and right hippocampi of control and P1VEH brains were stained with hematoxylin and eosin.
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