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Expression of MHC II, CD68, GFAP and gp41 in the brain were quantitated using 20 separate measures on each tissue sample; the mean was used for analyses.
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Arrays were quantitated using GenePix (http://www.moleculardevices.com).
Protein concentrations were quantitated using the Bradford assay (BioRad).
Bands were quantitated using the integrated density function of ImageJ.
Proteins were quantitated using the 2D Quant Kit (GE Healthcare).
Relative intensities were quantitated using MultiGauge software (Fujifilm).
Spots were quantitated using an AID ELISPOT High Resolution Reader System Cell Technology , Inc, Columbia, MD).
Results were quantitated using an ELx800UV spectrophotometer Bio-Tek Instrumentss). Bio-Tek Instruments
Signals were quantitated using a Fluorchem imager (Alpha Innotech).
Reactions were quantitated using a FACSCalibur flow cytometer.
Bands were quantitated using National Institute of Health's ImageJ software.
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