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Inflammatory infiltrates in the brain were quantified by haematoxylin and eosin (H&E) staining, as well as immunohistochemistry using antibodies against CD3, CD11b, and MMP-9 on days 20 after active immunization according to published methods [11], [24].
CCL2 concentrations in mouse and human brain were quantified by ELISA (Thermo Scientific) as described earlier [ 8].
To understand the effects of silagaba in vivo from the pharmacokinetic viewpoint, silagaba compounds were orally administered to rats and their concentrations in plasma and brain were quantified by LC-MS/MS.
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Aβ plaques in Drosophila brains were quantified by using ImageJ (NIH).
For each mouse, three sections per brain were quantified.
Transporter protein amounts in brain capillaries were quantified by liquid chromatography-tandem mass spectrometry.
Because Aβ38/42 and Aβ40/43 are distinct product/precursor pairs, these four species in the CSF together should faithfully reflect the status of brain γ-secretase activity, and were quantified by specific enzyme-linked immunosorbent assays in the CSF from controls and MCI/AD patients.
Neurofibrillary tangles in brain tissues from young and old fishes were quantified by analysis of confocal images acquired using the same procedure as for lipofuscin quantification.
The uptake of [11C]TASP0410457 in the brain was quantified with arterial blood sampling in anesthetized monkeys.
cDNA of each brain was quantified in duplicates.
NfH phosphoforms (SMI32, SMI34, SMI35) were quantified by ELISA from microdissected samples of control and MS brain and spinal cord.
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