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Adjacent 50 μm thick sections of each brain were pretreated with citrate buffer for 30 min at 65°C to increase antigen retrieval and penetration of the antibodies into the tissues.
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Briefly, 35 micrometer thick coronal brain sections were pretreated with 3% H2O2 in 0.1 M PBS for 30 min. The sections were rinsed in PBS twice for 5 min each.
Mice injected with gingipains developed degenerate brain cells, while mice that were pretreated with neutralizing compounds beforehand maintained healthy brain cells.
Furthermore, when C6 cells were pretreated with K252a, a TrkB antagonist, known to significantly inhibit the activity of brain-derived neurotrophic factor (BDNF), blocked the levels of phospho-ERK, phospho-Akt and phosphor-CREB.
Filters were pretreated with 0.1% BSA in Mg-HEPES.
All samples were pretreated with puromycin before RNA extraction.
Cows were pretreated with progesterone for 3 d.
After rats were pretreated with different concentrations of 4-MA, the neurologic scores, the infarct rate, and the EB contents in the brain tissues were significantly decreased.
To test this possibility, neutrophils were pretreated with TLR4 inhibitor before stimulated with OO extract.
HUVECs or RAW264.7 were pretreated with 0 uM or 20 uM FG-4592 before samples were collected.
Before the immunohistochemical staining, sections were pretreated with methanol/H2O series: 20%, 40%, 60 %, 80 %, 100 1 h each.
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