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For further verification, stimulation and iontophoresis sites within the brain were marked using electrical lesions (-4 μA DC for 5 min) through the electrodes implanted in the PAG and through the stimulation electrodes used to localize the vocally active sites in the PLA.
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Mitoses were marked using LongTracker.
Vessels were marked using immunohistochemistry with CD34.
Protein domains were marked using InterPro [ 103].
Duplicates were marked using Picard [ 28].
Agalychnis moreletii frogs were marked using PIT tags (Nonatec, United Kingdom).
The hybridization area was marked using a tipped scribe.
Volumes characterized by unusually high changes in volume to volume signal intensity (assessed using the mean squared signal change across the brain) were marked as bad volumes and replaced by interpolation of the volumes on either side.
AD brain is marked by obvious inflammatory features, in which microglial activation is the driving force.
NPC1-deficient brains are marked by neuronal lossand severe neurological symptoms.
MR gradient and pulse-related artifact were removed from the EEG 23, 24 using a commercial EEG processing package (Brain Analyzer; Brain Products), and IEDs were marked.
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