Sentence examples for brain were isolated using from inspiring English sources
Mononuclear cells from the lung and brain were isolated using the same procedure that was previously described for the spleen.
Hippocampal mitochondria from rat brain were isolated using a Percoll (Amersham Biosciences, Piscataway, NJ) gradient centrifugation as described previously.
Total RNA from NL and BDL rat brain was isolated using TRIzol® Reagent in the PureLink™ Micro-to-Midi Total RNA Purification System Invitrogen™ Life Technologiess, Foster City, CA, USA) according to the manufacturer's instructions.
RNA from individual brains was isolated using the Qiagen RNeasy Mini Kit with the optional on-column DNase treatment according to the manufacturer's instruction.
Total RNAs from postmortem adult human brain regions were isolated using the RNAwiz RNA isolation reagent and treated with DNase (Ambion, USA) according to the supplier's protocol.
For comparing gene expression patterns of the PRMS and DRMS, freshly dissected brains were cut with a vibratome (Leica, Wetzlar, Germany) in 100 μm sections, and the corresponding brain regions were isolated using a binocular microscope and shock frozen immediately after isolation.
GFP-L10a-containing ribosome-mRNA complexes were immunoprecipitated from ten pooled brain hemispheres, from which RNAs were isolated using Trizol (Invitrogen).
Proteins were isolated using Differential Detergent Fractionation (DDF) [ 27] for each of the tissues except muscle and brain.
Total RNAs were isolated using TRIzol (Invitrogen).
PBMCs were isolated using ficoll gradient centrifugation.
Total RNA from each brain sample was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and RNA was quantified by OD 260.
