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Hypothalamic glial cell cultures from 1-day postnatal brain were isolated following the method described previously [29], [48].
After incubation eggs were opened and either whole embryos or the embryonic brain were isolated.
Whole adult testis, thymus and brain were isolated from male mice and used for the analysis.
Mononuclear cells from the lung and brain were isolated using the same procedure that was previously described for the spleen.
Hippocampal mitochondria from rat brain were isolated using a Percoll (Amersham Biosciences, Piscataway, NJ) gradient centrifugation as described previously.
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A novel sequence, named MT-001, which shows enhanced expression in the permanent ischemic rat brain, was isolated by differential display.
One microgram of total RNA from adult fly brain was isolated using TRIzol reagent (Invitrogen), converted to complementary DNA using SuperScriptII reverse transcriptase (Invitrogen), and then used for quantitative RT PCR with SYBR Green reagent (Applied Biosystems).
Immediately following the last blood sample, the rats were euthanized, and the brain was isolated.
The ischemic area of the brain was isolated.
At the end of the experiments, mice were sacrificed, and the whole brain was isolated and frozen.
Total RNA from 5 porcine organs (inguinal lymph node, heart, spleen, lung, brain) was isolated with RNAprep pure Tissue Kit (TianGen Biotech (Beijing) Co., Ltd).
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