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28 axial slices (4 mm thickness, 0.5 mm skip) parallel to the anterior and posterior commissures covering the whole brain were imaged with a temporal resolution of 2 s using a T2* weighted gradient echo spiral pulse sequence: repetition time (TR) = 2000 ms, echo-time (TE) = 30 ms, flip angle = 80° and 1 interleave [28].
Interleaved axial slices covering the whole brain were imaged with a 1.8-mm isotropic spatial resolution (FOV = 23 × 23 cm, matrix = 128 × 128) using EPI single-shot spin-echo (SE) sequences (50 slices for infants; 70 for adults).
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At 24 h after the injection, tumor-bearing brain was imaged with the Maestro in vivo fluorescence imaging system (CRi Inc., Woburn, MA).
He says it's interesting that although the motor cortex, which controls movement itself, was active, the midline area of the brain, which is known to be activated when people are making a decision whether to move a limb, was not active in the lucid dreamer whose brain was imaged with fMRI.
Brains were imaged with a Zeiss LSM 510 confocal microscope.
The excised brains were imaged with SPECT using the same acquisition time as used for in vivo measurements.
Brain sections were imaged with a BioRad MRC 1024 confocal laser scanning system coupled to an Olympus IX-70 microscope.
Double or triple immunofluorescence-stained coronal brain sections were imaged with a Leica CTR5000 fluorescence microscope equipped with a Qimage RETIGA 2000R digital camera under 20× or 40× objectives.
To compare the fluorescence intensities and signal-to-noise ratios of RABV ∆G (VSV GRtmC), lentivirus- (LV), and adeno-associated virus (AAV) infected brain sections were imaged with identical microscopy settings.
Brain sections were imaged with a laser-scanning confocal microscope (LSM510-Meta; Carl Zeiss, Jena, Germany) with a × 63 (1.4 NA) PlanApo oil objective, a Nikon Ti-E inverted microscope with a × 60 (1.2 NA) PlanApo water objective, or a Keyence inverted microscope BZ-9000 with a × 10 (0.45 NA) CFI PlanApo λ objective.
Fixed brains were imaged by MRI with a 3D Diffusion Tensor Imaging (DTI) sequence consisting of a fast spin echo train length of 6 and a TR of 325 ms. TE for the first echo was 30ms, and then the echo spacing was set to 6 ms for the remaining 5 echoes.
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