Sentence examples for brain were fixed with from inspiring English sources

Twelve µm thick coronal cryosections of adult mouse brain were fixed with 4% paraformaldehyde in PBS and blocked with PBS containing 10% normal goat serum (Vector laboratories) and 0.3% Triton X-100.

The brain was fixed with 4% phosphate-buffered paraformaldehyde.

The brain was fixed with 4% paraformaldehyde and sliced into 100-μm-thick sections on a vibratome (Thermo Scientific, HM650V).

Brains were fixed with periodate lysine paraformaldehyde (PLP) fixative (McLean & Nakane 1974), immersed in 20% sucrose, embedded in OCT compound (Miles), and then frozen and sectioned coronally and tangentially (16 μm).

Briefly, brains were fixed with 4% PFA in PBS at 4°C overnight.

After 1 hour of administration, mice were sacrificed and brains were fixed with 4% paraformaldehyde for histological analyses.

Brains were fixed with 4% paraformaldehyde by transcardial perfusion and processed to produce 35um coronal cryostat sections.

Twenty-four hours after intraperitoneal injection of BrdU (100 mg/kg; Sigma-Aldrich Japan Inc., Tokyo, Japan), brains were fixed with 4% paraformaldehyde.

The brains were fixed with 2.5% Paraformaldehyde (PFA) for 2 3 hours, transferred to 15% sucrose solution with 1% PFA, and incubated at 4°C for not less than 16 hrs.

Fresh zebrafish brains were fixed with 4% paraformaldehyde at 4°C for 3 h, and then gradually immersed in PBS (0.09% NaCl in 0.1 M phosphate buffer) containing different concentrations of sucrose of 5 %, 10, and 20% and4°C.

Brains were fixed with 3%% glutaraldehyde in phosphate buffer.