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The total RNA of infected VeroE6 cells or mouse liver, spleen, kidneys and brain were extracted with Trizol reagent (Invitrogen).
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To enable prokaryotic expression of His-tagged L1ICD, including the amino acids Cys-Phe-Ile (CFI) of the transmembrane domain, the following steps were performed: RNA from murine brain was extracted with TRIzol according to the manufacturer's manual (Invitrogen, Karlsruhe, Germany).
The Evans blue in the minced brains was extracted with 500 μl of 4% paraformaldehyde for 2 days at 38 °C.
Total RNA from E12.5 brains was extracted with Trizol (Invitrogen) and further purified using RNAeasy purification columns (Qiagen).
Proteins in the animal brain homogenate were extracted with RIPA buffer.
RNAs from 90 glioma samples and 4 normal brain tissues (normal adjacent tissues) were extracted with Trizol reagent (Invitrogen, USA) accordingly.
They were measured and weighed before the neck was cut and the brain was extracted.
The brain was extracted and dissected into two hemispheres.
Brains were extracted as described before, postfixed with 4% PFA and cryoprotected with 30% sucrose.
The raw data were extracted with MarkerView.
Thirty minutes later, the animals were killed with carbon dioxide; the brains were extracted and snap frozen.
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