The movement of Eu-LVsp2 from blood to brain was examined using ratios that compare the counts per second per microgram of protein of whole brain or brain fraction (capillary depleted or capillary-enriched) relative to plasma as before [1].
Furthermore, the expression of p-PI3K in the hippocampus of the developing brain was examined using immunofluorescence.
All brains were examined using a Zeiss microscope equipped with a digital video camera (CCD, Optronics, Goleta, CA) and an image analysis system (Bioquant Nova, R&M Biometrics, Inc., Nashville, TN).
The morphology of the chicken brains was examined using hematoxylin-eosin staining.
The expression level of CaMKII after 4 h of glutamate treatment in the developing brains was examined using western blotting.
Brain sections were examined using a Leica DMIRE2 confocal microscope (Leica Microsystems, Exton, PA) with 63× oil objective and Leica Confocal Software (Leica Microsystems Heidelberg 19 GmbH).
Patients (n = 26) with brain metastases were examined using single voxel MRS at a 3T clinical MR system.
For these network analyses, the relationships between the Fos counts in different brain sites were examined using structural equation modelling (SEM).
Results are expressed as the mean±S.E.M. Brain sections were examined using a Leica DMIRE2 confocal microscope with the × 63 oil objective and Leica Confocal Software (Leica Microsystems, Wetzlar, Germany).
To determine whether APF formation is specific to certain cell types, DLB and PSP brain sections were examined using immunofluorescent labeling with αAPF and GFAP, MOG, or NeuN antibodies recognizing astrocytes, oligodendrocytes, and neurons, respectively.
