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Structural magnetic resonance images of brain were estimated by optimized voxel-based morphometry of FSL FMRIB Software Libraryy).
Biological effects of uranium on the brain were estimated by investigating the cholinergic pathway and oxidative stress.
The values for whole brain were estimated by adding together the levels of radioactivity and weights for all the brain regions.
The influence of these normalized eye movement variance values on theta and gamma power measurements at every voxel in the brain were estimated by linear regression.
Various inflammatory cytokines (that is, IL-1α and TNFα) and other inflammatory markers (that is, malondialdehyde, myeloperoxidase and nitric oxide) in serum and brain were estimated by ELISA, biochemical methods, and histopathology in both the experimental groups.
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Normal brain parameters were estimated by combining TMZ whole normal brain and brain microdialysis measurements as follows.
The protein levels for GLUD1 in brain homogenates were estimated by two different procedures, immunoblot assays following SDS/PAGE of brain proteins and ELISA of Glud1 in brain homogenates.
The OS, TTD, DFS, extracranial PFS and brain PFS were estimated by the Kaplan Meier product limit method.
Levels of DPP10789 in brain homogenates were estimated by immunoblot using the Quantity One (BioRad, USA) image analysis software package and were adjusted by the level of neuron specific protein β-tubulin.
Spastin levels from 4-month-old Sp+/+, Sp Δ/+ and Sp Δ/Δ mouse brain extracts were estimated by quantifying the immunoblot band density normalized to actin values (ImageJ software, NIH, Bethesda, MD) in three independent experiments.
Lipid peroxidation in brain was estimated colorimetrically by thiobarbituric acid reactive substances TBARS and hydroperoxides by the method of Niehius and Samuelsson [ 26] and Jiang et al. [ 27], respectively.
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