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Coronal sections of the entire brain were cut into 40-μm sections using a cryostat.
Coronal sections of the brain were cut into 40 50 μM thickness using a vibrating microtome (VT1000S, Leica, Milton Keynes, UK).
Samples of liver, heart and brain were cut into small pieces using a surgical blade, weighed and stored at −80°C until use.
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Each rat brain was cut into six sections and mouse brain into five sections.
In keeping with Vogt's method, each brain is cut into four or five chunks, which are encased in paraffin wax.
The brain was cut into 7 µm sections which were stained with hematoxylin and eosin (H&E stain).
For tissue analyses, the brain was cut into 10 µm coronal sections on a cryostat (Leica Microsystems).
The unfixed brain was cut into frontal slabs of approximately 10 mm thickness.
The brain was cut into slices (30 μm thick) using a cryostat and the sections were placed on glass slides.
The remaining brain was cut into 350 μm thick horizontal slices using a vibratome (Leica VT 1000S, Bensheim, Germany).
The anterior part of the brain was cut into coronal sections of 40 μm and stored for ChAT immunohistochemistry.
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