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Labelled cells on each side of the brain were counted using a 40X objective; only cells on the internal border of the subgranular zone of the dentate gyrus were included.
The numbers of GFP/NeuN double-positive cells in the brain were counted using a stereological method.
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Microglia in the cortex and striatum of Plp1tg 19–27 DPN and B6CBA 23–27 DPN brain sections were counted using a Leitz (Wetzlar, Germany) Laborlux microscope.
After the treatment, brains were mounted and photographed at 10X on a Zeiss Axioplan 2. GFP signals were counted using the Analyse Particle plugin in ImageJ.
Cell were counted using Visiopharm stereological software45.
Cells were counted using the ImageJ software.
Live cells were counted using a hemocytometer.
Stained nuclei were counted using ImageJ.
Cells were counted using ImageJ program (NIH).
Cells were counted using a Bürker hemocytometer.
Protoplasts were counted using a hemocytometer.
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