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After post-processing of the DTI white matter tracts of the brain were constructed using the Fibre Assignment by Continuous Tracking (FACT) algorithm [53].
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The head and brain templates were constructed using averaging techniques based on iterative methods that have become the norm for MRI template construction (Fonov et al., 2011; Sanchez et al., 2012a, b).
Brain-like structures were constructed using a bio-ink consisting of a novel peptide-modified biopolymer, gellan gum-RGD (RGD-GG), combined with primary cortical neurons.
For data from primary glioma specimens, expression heatmaps were constructed using expression data from the non-tumor brain specimens as a reference.
Phylogenetic trees were constructed using MEGA3 [73].
Kymographs were constructed using Metamorph 7.1.4 software.
Neighbor-joining trees were constructed using NEIGHBOR.
Figures and movies were constructed using CHIMERA [98].
Strains were constructed using standard genetic techniques.
Maps were constructed using maps2WinBUGS version 1.6.
Genome atlas visualisations were constructed using GENEWIZ [75].
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