First, most data regarding mitochondrial dysfunction in organs susceptible to failure in critical care diseases (liver, kidney, heart, lung, intestine, brain) were collected using animal models.
The mice were killed at 1, 7, and 30 days post exposure, and their organs, including heart, liver, spleen, stomach, kidneys, lungs and brain, were collected.
The organs including the liver, kidney, spleen, testis, and brain were collected from each rat, fixed with 10 wt.% formaldehyde solution at room temperature, embedded in paraffin block, sectioned, followed by staining with hematoxylin and eosin (H&E).
Those mice were, respectively, killed at No. 1-, 7- and 30-day post-injection, their organs such as heart, liver, spleen, stomach, kidneys, lungs, and brain were collected immediately.
At determined time points (0.5, 2, 4, and 6 h), the rats were sacrificed, and the tissues including the heart, liver, spleen, lung, kidney, and brain were collected, weighted, and frozen at −20 °C until further analysis.
Blood and brain were collected and plated to determine bacterial counts.
