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In this study, frozen cryostat sections from hippocampal and cerebellar regions of two rat strains and cerebellar and cerebral regions from a human brain were analyzed to determine the potential impact of these factors on estimates of neuron number obtained using the optical disector.
Sections at all levels of the brain were analyzed.
Six brains for each genotype and 16 18 sections per brain were analyzed.
For the sake of homogeneity, a total of 20 sections per brain were analyzed.
The 343 biomarker candidate genes for brain were analyzed with WebGestalt for tissue expression pattern and gene enrichment analyses.
In order to verify the specificity of transgene expression in families tg9 and tg41, total protein extracts from muscle, heart and brain were analyzed in western blot.
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The entire brain was analyzed and volumetric rendering technology was utilized, which enabled us to visualize the functional organization of intrinsic brain activity as volumetric source imaging.
At the age of 16 weeks five wild-type and five heterozygous Dll1tm1Gos/+ mutants were killed between 9 and 12 am by carbon dioxide and gene expression in liver, spleen, thymus and brain was analyzed as described previously [24].
Although the entire sectioned brain was analyzed for vascular leakage, the majority of blood vessel leakage was detected in the cerebellar lobules (6 9Cb), cerebral cortex (M2), hippocampus (CA1, CA2), and midbrain of mutant mice at all disease stages (Table 1).
Total RNA extracted from VEEV infected brain was analyzed by microarray as described in methods.
The cell death in Drosophila brain was analyzed as per the method described by Mitchell and Staveley [ 17].
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