Thirty-two translices slicoveringring the entire brain were acquired using a gradient-echo echo-planar pulse sequence (repetition time, TR = 2000 ms, echo time, TE = 30 ms, field of view, FOV = 220 mm, flip angle = 90°; matrix 64×64×32, spatial resolution, 3.4 mm×3.4 mm×3 mm).
32 transversal slices of functional images that covered the whole brain were acquired using a gradient-echo echo-planar pulse sequence (64×64×32 matrix with 3.4×3.4×4.4-mm spatial resolution, TR = 2000 ms, TE = 30 ms, FOV = 220 mm, flip angle = 90°).
24 axial slices (19.2 cm field of view; 64×64 pixel matrix; 4 mm thickness; 1 mm spacing; in-plane resolution of 3×3 mm) parallel to bicommissural line (AC-PC) covering the whole brain were acquired using a single-shot gradient echo-planar imaging (EPI) sequence (TR = 2000 ms; TE = 30 ms; flip angle = 90°; acquisition bandwidth = 100 kHz) sensitive to blood oxygenation level-dependent contrast.
T1-weighted anatomical images of the entire brain were acquired using a three-dimensional spoiled gradient echo pulse sequence (1.0 mm thick, matrix 256×256, field of view 22×22 cm).
High-resolution 3D T1-weighted axial images covering the entire brain were acquired using the following parameters: TR = 1900 ms, TE = 2.48 ms, slices = 176, thickness = 1 mm, gap = 0 mm, FA = 90°, acquisition matrix = 256 × 256, and FOV = 250 × 250 mm.
For the resting state functional MRI (fMRI), 34 transaxial gradient-echo images (64 × 64 matrix, TR = 2,000 ms, TE = 30 ms, flip angle = 70°, FOV = 24 cm, 3.75-mm slice thickness) covering the entire brain were acquired using an echo-planar sequence.
The fMRI time series data covering the entire brain was acquired using a T2-weighted gradient echo echo-planar imaging (GE-EPI).
A T1‐weighted structural image of the brain was acquired using a magnetization prepared rapid acquisition gradient echo sequence (field of view = 230mm, slice thickness = 0.9mm, repetition time [TR] = 1,900 milliseconds, echo time [TE] = 2.32 milliseconds, flip angle = 9°).
Images of brains were acquired using a Zeiss LSM510 confocal microscope.
Images of fixed zebrafish brains were acquired using a Leica fluorescence dissection microscope.
Images of brains were acquired using a Leica DM 5000B light microscope.
