Each functional image volume of the whole brain was acquired in 40 slices, each consisting of 64×64 voxels, of 3.5-mm thickness (no gaps between slices).
Three human brains were acquired in 2001 from the Department of Pathology at the University of California, Davis School of Medicine.
Subsequently, animals were placed in a 1 T Icon-Scanner (Bruker, Ettlingen, Germany) equipped with a mouse brain coil and T2-weighted images of the brain were acquired.
MDA-MB-231-Brain (brain-seeking) were acquired in the last two years from the National Cancer Institute, where they are regularly authenticated [MDA-MB-231-Brain cells were obtained from the NCI (Dr. Patricia Steeg) and were originally from Dr. Yoneda at UTSW].
Due to an error in the scanning protocol, for the first 14 scan sessions, brain slices were acquired in an interleaved manner, while the last 26 scan sessions were collected in an ascending order.
Dynamic brain PET data were acquired in 3D list-mode over 20 min.
Brain PET data were acquired in 3D mode over 5 min in one bed position, processed using standard correction methods, and reconstructed into a 256 × 256 matrix with 55 slices (voxel size 1.3 × 1.3 × 3.0 mm3) using 3D OSEM with four iterations, eight subsets, and a 5-mm Gaussian filter, resulting in PETPETCT for each patient.
> -wrap-foot> Brain MRI scans were acquired in all subjects on a 1.5 T GE Signa scanner (General Electric, Milwaukee, WI).
Whole-brain echoplanar functional images were acquired in 35 transverse slices (TR = 3000 ms, TE = 50 ms, matrix = 64 × 64, field of view = 192 mm, slice thickness = 3 mm, no gap, ascending interleaved order).
Mean numerical cell densities (N v ) of degenerating cells or total cell density (cells/mm) were acquired in 17 brain regions by means of a stereological dissector.
