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In order to rapidly and directly measure strain stability in BSE-infected brain, we utilized a method in which the fraction of PrPSc remaining at increasing GdnHCl concentration is measured with a commercial ELISA that specifically recognizes PrPSc (but not PrPC or denatured PrPSc).
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23 Briefly, to assess abnormally phosphorylated tau in the brain, we utilized three 50- μm thick coronal brain sections spaced 300 μm apart in all treated mice.
Instead, and to improve the amount of anatomical information gathered from neonatal brain imaging, we utilized a Diffusion Tensor Imaging (DTI) sequence, which provides the regional grey/white matter contrast, as previously employed by Zhang et al. (2006) [ 17].
Additionally, we utilized a human foetal brain cDNA library in a robot-based T7 phage display screening system with RA patient sera.
Here, we utilized an EEG-based mobile brain/body imaging (MoBI) technique that integrates high-density event-related potential (ERP) recordings with simultaneously acquired foot-force sensor data to monitor gait patterns and brain activity concurrently.
Recognizing the difficulties of obtaining normal brain tissue from children, we utilized the normal fetal brains and normal adult cerebral RNAs that were commercially available as references.
PET imaging can be used to quantify levels of amyloid in the brain by utilizing a radiotracer such as florbetapir (F-AV-45 or AV-45) or/and Pittsburgh compound-B (PiB, N-methyl-[C]- 40-methylaminophenyl -6-hydroxybenzothiazole).
Deep Brain Stimulation (DBS) utilizes a surgically implanted probe -- a brain "pacemaker" -- that stimulates brain regions instrumental to Parkinson's disease and depression.
We utilized brain-heart infusion (BHI) broth (Becton Dickinson, Sparks, MD) for culturing this strain and Miller's Luria Bertani (LB) broth (Mediatech Inc, Herndon, VA) for others.
We utilized whole brain high-resolution DTI combined with arterial spin labeling to quantify regional CBF on 15 healthy adults aged 23.2 57.1 years.
To confirm the expression of Lrp1b and Lrp4 ECDs, we utilized whole brain lysates and antibodies against the extracellular and intracellular ligand binding domains.
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