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To confirm the expression of miR-659 in human cells and in brain, we used a Taqman expression assay specific for miR-659 (Applied Biosystems).
For the entire brain we used a threshold of P<0.05 (FDR-corrected) with an extent threshold of k=10 voxels.
To help identify where our CRF microinjections actually acted in the brain we used a Fos plume mapping procedure to visualize spread of neuronal gene transcription triggered by 500 ng CRF in tissue around microinjection sites [ 43- 45].
To detect human glioblastoma cells in mouse brain, we used a 1 100 dilution of the mouse anti-vimentin antibody clone V9 (Dako, M0725), which has no cross-reactivity with mouse vimentin (Valadez et al., 2014).
In order to describe the general morphology of the remipede brain, we used a monoclonal antibody raised against acetylated α-tubulin from the sea urchin Strongylocentrotus purpuratus (Sigma, cat. no. T6793, lot no. 059 K4823, clone 6-11B-1 6-11B-1 6-11B-1
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In the adult mouse brain, we used an affinity chromatography approach, based on roscovitine immobilized on a solid matrix as previously described [9], [30].
To determine the localization of CB2-Rs in mouse and rat brains, we used a combination of Western blotting, immunohistochemistry and in-situ hybridization.
To test for a possible impact of parkin deficiency on dopaminergic neurons in the zebrafish brain, we used in situ hybridiziation of an antisense RNA probe for tyrosine hydroxylase (TH), which is the rate-limiting enzyme in the synthesis of dopamine.
To analyse the spatial distribution of DMD gene expression across the adult brain, we used the Allen Human Brain Atlas (AHBA), which has a much higher resolution than the BrainSpan atlas but lacks the temporal dimension.
In 2010, researchers at McGill University in Montreal showed that a reliance on GPS might reduce activity in the hippocampus, a part of the brain we use to get from A to B. Another study, from Columbia University in New York, investigated the "Google effect" and found that people used computers as a substitute for their own memories.
To avoid the effects of anesthesia or stress, which are known to elicit Fos induction in various brain regions, we used a novel thermode specifically designed for chronic warming of discrete brain structures in freely moving rats.
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