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Human brain was utilized as a positive for both TREK-1 and TRAAK protein expression.
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Closure strategies were utilized as necessary.
Descriptive statistics were utilized as appropriate.
As an example, Table 1 provides threshold criteria used for each method for subject 5. Since the true hub classification is not known for brain networks, functional cartography was utilized as an alternate to centrality measures.
When the specific reverse complementary sequence to the cIRK1 5'UTR at positions 61 41 was utilized as a primer for reverse transcription, a product of approximately 80 bases was generated from brain mRNA (Fig. 1C).
Hprt1 was utilized as internal control.
β-actin was utilized as internal control.
Under normal conditions, the concentration of KBs is very low (<0.3 mmol/l) compared with glucose (∼4 mmol), and as glucose and KBs have a similar kM for glucose transport to the brain the KBs begin to be utilized as an energy source by the CNS when they reach a concentration of about 4 mmol/l, which is close to the Km for the monocarboxylate transporter.
Proteins such as cardiac troponin (cTn), creatine-kinase myocardial band isoform (CK-MB), brain natriuretic peptide (BNP), and its N-terminal prohormone (NT-proBNP) have been utilized as biomarkers to detect and monitor myocardial damage [ 3].
In another experiment, their brains were utilized for PCR analysis of nestin and Sox2.
The resonance characteristics arising from quantum effects in nano-scale devices are utilized as computing primitives of correlation and employed in building brain-mimicking VLSI systems.
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